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Solid-phase microextraction of volatile organic compounds in headspace of PM-induced MRC-5 cell lines.
J. Li, N. Chen, Y. Tian, H. Xu
Talanta 2018 Aug;185:23-29.
PubMed: 29759194
Abstract
The exploration of volatile organic compounds (VOCs) produced by cell lines may be a powerful and non-invasive tool for the study of the health risk of human exposure to atmospheric particulate matter (PM). In this work, we developed a sensitive solid phase microextraction-gas chromatography-mass spectrometry method (SPME-GC-MS) to analyze VOCs in breathed gas of PM2.5-induced human embryonic fibroblast cell line (MRC-5). A novel graphene oxide/polyaniline/polydopamine (GO/PANI/PDA) coating was prepared on a stainless steel wire via electrochemical deposition and self-polymerization for the first time. The GO/PANI/PDA coating exhibited high extraction efficiency, good thermal stability (> 380 ℃), excellent mechanical stability as well as long service time (> 150 times). Parameters that may affect the results were optimized systematically. Under the optimal conditions, VOCs including benzene series, aldehydes and alkane were detected with low limit of detection (0.2-2.0 μg L-1) and good correlation (correlation coefficients above 0.9922). The relative standard deviations of within-day and between-day were 1.1-8.4% and 0.2-11.2%, respectively. Satisfactory recoveries of 82-117% indicated good repeatability of the method. The method has been successfully applied for the determination of target VOCs in the headspace gas of PM2.5-induced MRC-5 cell. And it is expected to provide an alternative tool for the study of cytotoxicology of atmospheric particulates.
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No associated compound. |