Back
Detection of specific Helicobacter pylori DNA and antigens in stool samples in dyspeptic patients and healthy subjects.
M. Wiśniewska, HO. Nilsson, L. Bak-Romaniszyn, T. Rechciński, W. Bielański, I. Płaneta-Małecka, M. Płonka, S. Konturek, T. Wadström, W. Rudnicka, M. Chmiela
Microbiol. Immunol. 2002 ;46(10):657-65.
PubMed: 12477244
Abstract
In this study stool samples from dyspeptic patients and healthy subjects were used for detection of specific Helicobacter pylori antigens and DNA by immunoenzymatic test (PPHpSA) and semi-nested PCR (ureA-PCR), respectively. The H. pylori status was estimated by invasive endoscopy-based rapid urease test and histology or noninvasive urea breath test (UBT), and by serology (ELISA, Western blot). The coincidence of H. pylori-negative invasive tests or UBT and negative antigen or DNA stool tests was very high (mean 95%). The PPHpSA results were found positive for 56% and ureA-PCR for 26% of individuals with H. pylori infection confirmed by invasive tests or UBT. The detection of specific H. pylori antigens and especially DNA in feces is not sufficient as a one-step diagnosis of H. pylori infection.
Associated compounds:
Compound Name
with link to compound page |
Structure | Number of references |
---|---|---|
Urea | 1130 |